Abstract
Adult rat hepatocytes in primary culture responded to epidermal growth factor (EGF) by increased DNA synthesis. When hepatocytes were cultured in Leibovitz L-15 medium, hepatocyte response to EGF was low compared with those cultured in Williams'medium E or Koga's medium L. Furthermore, in L-15 medium, female rat hepatocytes showed almost no response to the mitogenic action of EGF compared with male rat hepatocytes. Addition of glutamic acid (1 to 20 mM) to EGF-containing L-15 medium not only enhanced DNA synthesis over 10-fold in both male and female hepatocytes, but eliminated the sex differences in DNA synthesis. Glutamic acid by itself did not stimulate DNA synthesis at any concentrations tested
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