Abstract

A procedure for classifying histochemically stained skeletal muscle fibers using a personal computer was established. Frozen sections of rat rectus abdominis muscle were stained for myosin ATPase with preincubation at low and high pH, and for a mitochondrial enzyme, NADH tetrazolium reductase. Light microscopic images of the sections were converted through a video camera into computerized images which were then analyzed with digital image-processing software in a personal computer to recognize individual muscle fibers and to categorize them into slow-twitch, oxidative type; fast-twitch, oxidative-glycolytic type; and fast-twitch, glycolytic type

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