Abstract
In this experiment a total of 300 d-old broilers (Ross 308) were randomized across 20 floor pens and reared up to 42 d. Treatments includes: 1) A normal-protein diet (NPD) was formulated according to the Ross 308 recommendation (2014) by corn and soybean meal, 2) A reduced-protein diet (RPD) was prepared with dietary protein reduced by 12 g kg -1 (1.2%) relative to the NPD, 3) HMB+ RDP, 4) CLA+RDP and 5) CLA+ HMB+ RDP. The results showed that broilers fed HMB diet had higher Average Daily Gain (ADG) and Feed Conversion Rate (FCR) significantly improved by HMB during the grower or the whole period (p < 0.05). Titer of serum antibodies of Newcastle disease was maximum in treatment supplemented with CLA+HMB compared to the control (RPD) in day 42. In comparison with the control, feeding CLA increased Lymphocyte and decreased heterophil and H/ L ratio (p < 0.05). Insulin hormone secretion increased by dietary HMB (p < 0.05). Red Blood Cells (RBC) and Hemoglobin (Hb) was significantly increased by HMB supplemented diet (p < 0.05). It could be concluded that supplementations of HMB and CLA together in reduced-protein diet have positive effect on growth performance, immune system and hematological parameter.
Highlights
Efficient use of nutrients in order to maximize production of poultry meat is one of the most important goals of poultry nutrition science (Khoramabadi, Akbari, Khajali, Noorani, & Rahmatnejad, 2014)
The nutritional principle involved is to use of dietary supplements such as β-Hydroxy-β-methyl Butyrate (HMB) and Conjugated Linoleic Acid(CLA) that protein and energy may be used with high efficiency and direct it towards growth performance
The average daily gain (ADG) was increased in the finisher stage and throughout the feeding trial with HMB compared with the negative control (RPD) (p < 0.05)
Summary
Efficient use of nutrients in order to maximize production of poultry meat is one of the most important goals of poultry nutrition science (Khoramabadi, Akbari, Khajali, Noorani, & Rahmatnejad, 2014). The reduction of crude protein (CP) in diets, properly supplemented with crystalline amino acid, could help to reduce nitrogen excretion in feces, urine and litter quality (Khajali & Wideman, 2010). It is reducing the energy expenditure associated with the excretion of excess of dietary CP. The nutritional principle involved is to use of dietary supplements such as β-Hydroxy-β-methyl Butyrate (HMB) and Conjugated Linoleic Acid(CLA) that protein and energy may be used with high efficiency and direct it towards growth performance. The objective was to evaluate HMB and CLA supplementation in reduced protein diet on grows performance, immune system and hematological index in broiler chickens
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