Abstract
Biomass from lignocellulosic material constitutes a promising energy alternative and without competing with food production. However, pretreatments are required for conversion into sugars which release hexoses, pentoses and other sugars, coupled to inhibitors. Current analysis focuses on ethanol production with the three major inhibitors of lignocellulosic biomass pretreatment, namely, acetic acid, furfural and 5-hydroxymetilfurfural (HMF), and investigates the influence of a mixture of these inhibitors on fermentation by Pichia stipits , using commercial xylose as the only carbon source, through a full factorial 2 3 + 3 design of experiments (DOE). Fermentations were conducted in a laboratory scale, at 150 rpm and 72h, in a complex culture media with xylose and different inhibitor concentrations, based on the experimental analysis of sugarcane bagasse and 2.10 7 cell mL -1 of initial concentration of the microorganism. Experimental results showed a significant influence of acetic acid concentration, which must be at the lowest possible level, with no influence of furfural and hydroxymethyl furfural respectively up to concentrations 2.25 and 0.75 g L -1 .
Highlights
In 2012, Brazil produced 23.54 million cubic meters of ethanol (Agência Nacional do Petróleo, Gás natural e Biocombustíveis [ANP], 2013) by conversion of sucrose from sugar cane
The use of biomass enables the development of new technologies with the consequent reduction of economic and environmental impacts (Balat, 2001; Chemmés, Silva, Souza, Azevedo, & Campos, 2013), in which lignocellulosic residues are highlighted as renewable sources for bioethanol production
A mixture of the three major inhibitors produced during lignocellulosic biomass treatment, acetic acid, furfural and 5-hydroxymethyl furfural, were evaluated on P. stipitis fermentation, with regard to biomass growth, xylose uptake, ethanol yield and productivities by a full factorial design 23 with triplicates on central point, totaling 11 ethanolic fermentation experiments
Summary
In 2012, Brazil produced 23.54 million cubic meters of ethanol (Agência Nacional do Petróleo, Gás natural e Biocombustíveis [ANP], 2013) by conversion of sucrose from sugar cane. The use of biomass enables the development of new technologies with the consequent reduction of economic and environmental impacts (Balat, 2001; Chemmés, Silva, Souza, Azevedo, & Campos, 2013), in which lignocellulosic residues are highlighted as renewable sources for bioethanol production. They do not compete with the food industry and represent the most abundant carbohydrate reserves in the world (Saha, Yoshida, Cotta, & Sonomoto, 2013).
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