Abstract
The major histocompatibility complex (MHC) is a set of genes found on the short arm of chromosome 6. MHC molecules in human beings are known as human leukocyte antigens (HLA). HLA polymorphism can be determined by serological and molecular typing methods, which may yield discordant results. The present analysis performed HLA typing of samples with discordant results by PCR- SSP and PCR-SSO, so that typing discrepancies could be clarified. The cross-sectional study analyzed 33 samples from individuals included in an HLA-disease association study. Discrepant alleles were observed in 6 of 33 samples. Discordant samples were retyped using One Lambda Micro SSP™, Dynal RELI™ SSO and Luminex™ SSO assays for HLA class I (HLA-A, HLA-B) and class II (HLA-DRB1) molecules. The three methods produced concordant results after HLA retyping. Human error occurred in interpreting the initial results, which led to discrepancies in the results obtained. The participation of experienced professionals and the availability of at least two different methods to confirm doubtful or inconclusive results are mandatory for effective HLA typing.
Highlights
The major histocompatibility complex (MHC) is a set of genes found on the short arm of chromosome 6
The first sample was typed as human leukocyte antigens (HLA)-A*24, A*30 by polymerase chain reaction with sequence-specific primers (PCR-SSP) and as HLA-A*24, A*32 by PCR-sequence-specific oligonucleotide (SSO)
The second sample was typed as HLAA*03, A*30 by PCR-SSP and as HLA-A*02, A*30 by PCR-SSO
Summary
The major histocompatibility complex (MHC) is a set of genes found on the short arm of chromosome 6. Currently the method of choice in histocompatibility laboratories, provides better resolution than serological typing. The present study was conducted when discrepancies in HLA typing results were observed Maringá, v. 11-14, Jan.-June, 2014 during an HLA-disease association study conducted in the same laboratory and by independent research groups, hereinafter referred to as group X and group Y. Group X conducted an association study using the Micro SSPTM assay (One Lambda®) for HLA-A, -B and -DRB1 typing. Group Y initiated another HLA-disease association study and used Dynal RELITM SSO for HLA typing. During HLA typing, samples from some patients used by both research groups (groups X and Y) showed discrepant HLA typing results. The present analysis performed HLA typing of samples with discordant results, using three different methods so that typing discrepancies could be clarified
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