&lt;b&gt;Cryopreservation of the mangaba tree (&lt;em&gt;Hancornia speciosa&lt;/em&gt; Gomes): a protocol for long-term storage

Paulo Augusto Almeida Santos,Ana Cristina De Souza,Diogo Pedrosa Corrêa Da Silva,Marlucia Cruz De Santana,Renato Paiva,Luciano Coutinho Silva

doi:10.4025/actasciagron.v37i3.19573

Abstract

The aim of this study was to evaluate the efficiency of vitrification and droplet vitrification for the cryopreservation of Hancornia speciosa shoot tips. The shoot tips were subjected to four different periods of exposure (15, 30, 45 and 60 min.) to plant vitrification solution 2 (PVS2) before plunging into liquid nitrogen. We evaluated the regrowth of H. speciosa shoot tips that were cryopreserved by the classical vitrification technique and by the droplet vitrification technique. Shoot tips were submitted to different periods of pre-culture (absence, 24 or 48h) in a medium containing 0.3 M sucrose prior to cryopreservation. With a PVS2 exposure period of 60 min., significant differences were observed between the two techniques used in this study with respect to regrowth rates; however, both cryopreservation methods allowed for a shoot tip regrowth rate of over 70%. Use of a preculture step increased the regrowth rate of cryopreserved shoot tips. Shoot tip cryopreservation techniques employing vitrification and droplet vitrification proved to be viable for the long-term storage of mangaba tree samples.

Highlights

Mangaba tree (Hancornia speciosa Gomes) is a native Brazilian fruit tree occurring in the Atlantic Forest and Cerrado
Because there is no established protocol for longterm conservation of H. speciosa, the aim of this study was to evaluate the efficiency of two cryopreservation techniques—the classical vitrification method and droplet vitrification—for H. speciosa shoot tip preservation in liquid nitrogen
After 30 days of cultivation, we evaluated shoot tip regrowth and callus formation

Summary

Introduction

Mangaba tree (Hancornia speciosa Gomes) is a native Brazilian fruit tree occurring in the Atlantic Forest and Cerrado. This species has economic potential due to its fruits, which are consumed fresh and used to produce juices, sweets and ice creams (MOURA et al, 2011). Recent studies have shown that the leaf extract has anti-hypertensive and vessel dilator effects (PEREIRA et al, 2012), whereas the latex has anti-inflammatory properties. Agronomy (MARINHO et al, 2011). These recent findings indicate the medicinal potential of this species. 289-296, July-Sept., 2015 means of ensuring the in vitro conservation of species diversity (SANTOS et al, 2011) Micropropagation studies are needed to allow the proliferation of genotypes (SÁ et al, 2012) and to provide new Maringá, v. 37, n. 3, p. 289-296, July-Sept., 2015 means of ensuring the in vitro conservation of species diversity (SANTOS et al, 2011)

Objectives

Methods

Results