Abstract
Byrsonima intermedia is a shrub from the Brazilian Cerrado with medicinal properties. The storage of biological material at ultra-low temperatures (-196°C) is termed cryopreservation and represents a promising technique for preserving plant diversity. Thawing is a crucial step that follows cryopreservation. The aim of this work was to cryopreserve B. intermedia zygotic embryos and subsequently thaw them at room temperature in a solution rich in sucrose. The embryos were decontaminated and desiccated in a laminar airflow hood for 0-4 hours prior to plunging into liquid nitrogen. The embryo moisture content (% MC) during dehydration was assessed. Cryopreserved embryos were thawed in a solution rich in sucrose at room temperature, inoculated in a germination medium and maintained in a growth chamber. After 30 days, the embryo germination was evaluated. No significant differences were observed between the different embryo dehydration times, where they were dehydrated for at least one hour. Embryos with a MC between 34.3 and 20.3% were germinated after cryopreservation. In the absence of dehydration, all embryos died following cryopreservation. We conclude that B. intermedia zygotic embryos can be successfully cryopreserved and thawed at room temperature after at least one hour of dehydration in a laminar airflow bench.
Highlights
The Brazilian Cerrado shelters numerous plant species with high fruitful and/or medicinal potentials
The Cerrado harbours the largest savannah plant biodiversity on the planet (KLINK; MACHADO, 2005), with more than 10,000 plant species (RATTER et al, 1997), which is persistently threatened by the intensive agricultural use of the
Juss. is a medicinally valuable species that belongs to the Malpighiaceae family from the Cerrado, and, such as numerous species of this genus, it presents seeds with a coat
Summary
The Brazilian Cerrado shelters numerous plant species with high fruitful and/or medicinal potentials. The Cerrado harbours the largest savannah plant biodiversity on the planet (KLINK; MACHADO, 2005), with more than 10,000 plant species (RATTER et al, 1997), which is persistently threatened by the intensive agricultural use of the. Studies on the propagation of native species are scarce, and numerous species that represent an ecological and/or pharmaceutical interest could become extinct before being properly studied. Is a medicinally valuable species that belongs to the Malpighiaceae family from the Cerrado, and, such as numerous species of this genus, it presents seeds with a coat Juss. is a medicinally valuable species that belongs to the Malpighiaceae family from the Cerrado, and, such as numerous species of this genus, it presents seeds with a coat
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