Abstract
Histamine-evoked changes in catecholamine (CA) secretion and intracellular free Ca 2+ concentration, [Ca 2+ ] i , in the perfused rat adrenal medulla were analyzed by real-time monitoring of CA release and by microfluorometry of fluo-3-loaded adrenal medullae, respectively. Histamine-evoked CA secretion consisted of at least three components; the first component formed initial transient secretion which was insensitive to a decrease in external Ca 2+ concentration, [Ca 2+ ] o , to 16 μM and was inhibited by an intracellular Ca 2+ antagonist, TMB-8
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