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Abstract
Endophytic bacteria isolated from Eichhornia crassipes (Mart) Solms., collected in oil contaminated wastewater of effluent generated by Petrobras refinery in Manaus were investigated to determine their potential for producing biosurfactants. Assay with 2.6-dichlorophenol indophenol (DCPIP) indicator to verify hydrocarbon biodegradation activity; oil emulsification test; drop-collapse method; surface tension and growth curve of biosurfactant production. The M87 Microbacterium sp. strain chosen for this work was identified by the sequencing of the rDNA region and the chemical characterization was performed by FTIR, UFLC/MS and 1H RMN techniques. The selected bacterial isolate provided 3g L -1 of biosurfactant, using diesel oil as sole carbon source, being efficient in biodegrading oil as demonstrated by the DCPIP test. Fractions obtained by column chromatography were efficient in reducing water surface tension around 40 mN m -1 , especially fraction 1, which reduced it to 34.17 mN m -1 . The different techniques of chemical analysis used for the identification of the biosurfactant isolate indicated that this is probably a long - chain fatty acid lipid type, which may be used in the future as both biosurfactant in decontamination processes of hydrocarbon-polluted areas or as bioemulsifier in countless processes, since it exhibited no toxicity as determined by Alamar Blue assay.
Highlights
Microbial biosurfactants have potential commercial applications within several industrial sectors
M87 Microbacterium sp. strain isolated from Eichhornia crassipes (Marts.) collected in oil contaminated wastewater of effluent generated by the Petrobras refinery in Manaus (REMAN)
The sequences were aligned and edited with MEGA6 software (Tamura, Stecher, Peterson, Filipski, & Kumar, 2013) and grouped by NeighborJoining method, and used to identify the isolates by comparing them with the patterns based on the results found in BLASTn
Summary
Microbial biosurfactants have potential commercial applications within several industrial sectors These products are effective in enhancing oil recovery and in the bioremediation of hydrocarbon-contaminated environments. Biological Sciences pharmaceuticals, personal hygiene and food processing products, among others (Colla & Costa, 2003, Sourav, Susanta, Ghosh, Saha, & Saha, 2015). This work represents an important contribution for the identification of bacterial isolates collected from oil-contaminated environments in the Amazon region. Those strains can further be exploited commercially, both for the production of new bioemulsifiers or biosurfactants and for the bioremediation of oil-contaminated environments. Crassipes (Marts.) was evaluated and the chemical characterization of the biosurfactants was determined
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