&lt;b&gt; Assessment of petroleum biodegradation for &lt;i&gt;Bacillus toyonensis&lt;/i&gt; by the using redox indicator 2,6 dichlorophenol indophenol

Ferdyanne Beatriz Sabóia Peixoto,Diana Cibelli Lucas Motta,Spartaco Astolfi-Filho,Jean Charles Da Cunha Peixoto,José Odair Pereira,Ana Teresa Miranda Peixoto

doi:10.4025/actascibiolsci.v40i1.35640

<b> Assessment of petroleum biodegradation for <i>Bacillus toyonensis</i> by the using redox indicator 2,6 dichlorophenol indophenol

Ferdyanne Beatriz Sabóia Peixoto, Diana Cibelli Lucas Motta + Show 4 more

Open Access

https://doi.org/10.4025/actascibiolsci.v40i1.35640

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Abstract

Petroleum degrading microorganisms have been isolated from different environments with the purpose of being used in bioremediation processes in areas impacted by petroleum spills. The objective of this study was to evaluate the ability of Bacillus toyonensis AM07 strain to metabolize petroleum compounds. The strain was isolated from the effluent dike of the Urucu Petroleum Province, Coari - Amazonas, Brazil. The degrading activity of B. toyonensis was evaluated by the colorimetric method using the redox indicator 2,6-dichlorophenol indophenol (DCPIP). Thus, the microorganism was inoculated into minimal medium with DCPIP, and with petroleum as the sole carbon source. The degradation potential of the microorganism was found by changing the DCPIP staining and absorbance readings 600 nm . The results obtained demonstrated that the bacterial strain was able to degrade petroleum by altering the color of the medium from blue to colorless and by reducing the concentration of the indicator in the absorbance readings. B. toyonensis AM07 strain has shown good performance in the petroleum degradation assays and may be used in the future in remediation technologies for hydrocarbon impacted environments.

Highlights

The microorganisms capable of degrading petroleum have been isolated from different environments with the objective of being used in bioremediation processes of impacted areas (Olajire, & Essien, 2014; Das, & Chandran, 2011)
The bacterial strain AM07 is deposited in the culture collection of the Laboratory of Bacterial Genetics of Universidade Federal do Amazonas - UFAM, Manaus
The biodegradability test using dichlorophenol indophenol (DCPIP) is a low complexity and low-cost test with proved efficiency by different authors, which highlight the important role of this indicator in the detection of microorganisms, such as fungi, bacteria and yeasts with the potential for degrading petroleum and derivatives (Hanson et al, 1993; Bidoia et al, 2010; Luz, et al, 2011; Mariano, Bonotto, De Angelis, Pirôllo, & Contiero, 2008)

Summary

Introduction

The microorganisms capable of degrading petroleum have been isolated from different environments with the objective of being used in bioremediation processes of impacted areas (Olajire, & Essien, 2014; Das, & Chandran, 2011). The species of the genus Bacillus have been reported as petroleum compounds degraders, such as pyrene, naphthalene and n-alkenes (Darsa et al, 2014; Sorkhoh, Ibrahim, Ghannoum, & Radwan, 1993). In this genus, a new specie was described by Jiménez et al (2013a) as part of the phylogenetic group Bacillus cereus, named B. toyonensis. Biological Sciences, v. 40, e35640, 2018 weihenstephanensis, B. toyonensis and Bacillus cytotoxicus (Jiménez, Blanch, Tamames, & Rosselló-mora, 2013b)

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