&lt;b&gt;Aquatic microbiota diversity in the culture of Nile tilapia (&lt;i&gt;Oreochromis niloticus&lt;/i&gt;) using bioflocs or periphyton: virulence factors and biofilm formation

Jéssica Lucinda Saldanha Da Silva,Fátima Cristiane Teles De Carvalho,Regine Helena Silva Dos Fernandes Vieira,Oscarina Viana De Sousa,Marcelo Vinícius Do Carmo E Sá,Davi De Holanda Cavalcante

doi:10.4025/actascianimsci.v38i3.31910

<b>Aquatic microbiota diversity in the culture of Nile tilapia (<i>Oreochromis niloticus</i>) using bioflocs or periphyton: virulence factors and biofilm formation

Jéssica Lucinda Saldanha Da Silva, Fátima Cristiane Teles De Carvalho + Show 4 more

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https://doi.org/10.4025/actascianimsci.v38i3.31910

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Abstract

The following research isolated and identified the main bacterial groups present in the culture of juvenile Nile tilapia in the presence of bioflocs and/or periphyton. The strains were also tested for the production of exoenzymes, indicative of potential virulence factors, and ability to form biofilm. The water samples were taken from tilapia cultured in the presence of bioflocs (T1), in the presence of bioflocs and periphyton (T2), from traditional culture (T3) and from culture in the presence of periphyton (T4). In the growth and selection of the bacterial groups, pour plate method was used, along with the following media: Plate Count Agar (PCA - DIFCO), Aero Pseudo Selective Agar (GSP - Himedia) and Nutrient Agar (AN - Merck). 46 strains were isolated in the following distribution: T1 (n = 12); T2 (n = 10); T3 (n = 14) and T4 (n = 10). Among the isolates, the most frequent genera were: Pseudomonas spp., Aeromonas spp., Staphylococcus spp., Bacillus spp., Mycobacterium spp., Micrococcus spp., and Corybacterium spp. Bacterial isolates in treatments T1 and T3 tested positive for five virulence profiles each, while those isolated from T2 and T4 for two and three virulence profiles, respectively. Treatments in bioflocs and periphyton (T2) or only periphyton (T4) yielded bacteria of less pathogenic potentials. In relation to the fish growth, T1 and T4 resulted in a higher final weight.

Highlights

Aquaculture is the food industry sector that presents the highest growth rate (Food and Agriculture Organization [FAO], 2014), Acta Scientiarum
Survival rate of the fish was higher than 80% and their final weight was, in average, 7.79 ± 0.82, 28.14 ± 2.7, 24.35 ± 1.04, 26.04 ± 2.07 for Treatment 1 (T1), Treatment 2 (T2), Treatment 3 (T3) and Treatment 4 (T4), respectively
It is noticeable that the presence of bioflocs (T1 and T2) and of periphyton (T4) contributed to a higher final weight in comparison to T3

Summary

Introduction

Aquaculture is the food industry sector that presents the highest growth rate (Food and Agriculture Organization [FAO], 2014), Acta Scientiarum. Animal Sciences complementing current deficits in fishing. In Brazil, tilapia was the most produced freshwater fish (Brasil, 2014). Different culture systems create an artificial environment which are able to directly influence microorganisms, increasing the selection, adaptation and growth of specific bacterial communities in regular aquatic microbiota (Jiravanichpaisal, Miyazaki, & Limsuwan, 1994; Aguirre-Guzmán, Ruíz, & Ascencio, 2004). A number of diseases might affect tilapia production, generating high mortality rates and, economic losses. Among the main pathogenic bacteria in the culture of tilapia are Flavobacterium columnaris, Aeromonas sp., Vibrio sp., Streptococcus iniae, S. agalactiae, Edwardisiella tarda, Francisella sp. Among the main pathogenic bacteria in the culture of tilapia are Flavobacterium columnaris, Aeromonas sp., Vibrio sp., Streptococcus iniae, S. agalactiae, Edwardisiella tarda, Francisella sp. (Kubtiza, 2008; Soto, Hawake, Fernandez, & Morales, 2009)

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