Abstract
The biological activity of honey has been the subject of great scientific investigation. Bee products are widely used in folk medicine to treat human diseases due to their antibacterial and inhibitory potential action on different human pathogens. Ten honey samples produced by Apis mellifera in the northern of Minas Gerais state were tested against pathogenic microorganisms Staphylococcus aureus TSST (clinical isolated) and enterohaemorrhagic Escherichia coli ATCC 43895 for determination of their minimum inhibitory concentrations. The microdilution technique in broth Mueller-Hinton broth (MHB) was used in four concentrations (25, 12.5, 6.25 and 3.125%). There was a reduction of bacterial growth for the two target species at all concentrations tested. The optimal concentration for inhibition of S. aureus and E. coli were 3.125% (w/v). Therefore, the antibacterial activity of the tested samples evidences the potential of Aroeira honey produced in the north of Minas Gerais for therapeutic use, thus contributing to the aggregation value and commercialization of this type of honey.
Highlights
Honeys are complex substances produced by bees and may contain different compounds with bioactive properties, which vary according to the region where they are produced and collected (PitaCalvo & Vásquez, 2017)
The objective of this work was to determine the minimum inhibitory concentration of honeys produced by Apis mellifera bees in the north of Minas Gerais against pathogenic microorganisms of clinical importance S. aureus TSST and E. coli (EHEC) cause of hemorrhagic diarrhea
The microorganisms were grown in Petri dishes containing Mueller-Hinton agar (MHA) and incubated at 37°C for 18 to 20 hours before to antimicrobial inhibition assays for purity and viability observation
Summary
Honeys are complex substances produced by bees and may contain different compounds with bioactive properties, which vary according to the region where they are produced and collected (PitaCalvo & Vásquez, 2017). The Aroeira honey is produced by Apis mellifera bees in the Brazilian Dry Forest. It is characterized by predominance of Myracrodruon urundeuva and in this biome, the scarcity of floral resources, associated with high temperatures and low humidity, induce bees to seek these massive food sources to ensure their food supply. This leads to the productions of a honey which has peculiar characteristics that
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