Abstract

This is an experimental, analytical and numerical study to optimize the biosurfactants production in solid-state fermentation of a medium containing rice straw and minced rice bran inoculated with Aspergillus fumigatus. The goal of this work was to analytically model the biosurfactants production in solid-state fermentation into a column fixed bed bioreactor. The Least-Squares Method was used to adjust the emulsification activity experimental values to a quadratic function semi-empirical model. Control variables were nutritional conditions, the fermentation time and the aeration. The mathematical model is validated against experimental results and then used to predict the maximum emulsification activity for different nutritional conditions and aerations. Based on the semi-empirical model the maximum emulsification activity with no additional hydrocarbon sources was 8.16 UE·g-1 for 112 hours. When diesel oil was used the predicted maximum emulsification activity was 8.10 UE·g-1 for 108 hours.

Highlights

  • Biosurfactants are active compounds produced at the microbial cell surface or excreted and which reduce surface and interfacial tension producing emulsification (ILORI et al, 2005)

  • This paper presents an experimental, numerical and analytical study of the optimal nutritional conditions for maximum biosurfactants production in solid-state fermentation by Aspergillus fumigatus

  • Experimental results published by Veenanadig et al (2000) indicated that biosurfactants production by Bacillus subtilis SSF in fixed bed column bioreactors has increased with the aeration increase

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Summary

Introduction

Biosurfactants are active compounds produced at the microbial cell surface or excreted and which reduce surface and interfacial tension producing emulsification (ILORI et al, 2005). Based on their characteristics, the biosurfactants are useful in remediation of insoluble organic pollutants in soil and marine environments (MARTINS et al, 2006). Technology nutrients needed to obtain high production yields. The optimizing of these factors that affect growth in biosurfactant producing organisms with potential for commercial exploitation is of major importance (SOUMEN et al, 2006)

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