Abstract
This paper reports our original technique for visualizing cell-attached nanointerfaces with extremely high axial resolution using homogeneously excited localized surface plasmon resonance (LSPR) on self-assembled silver nanoparticle sheets. The LSPR sheet can confine and enhance the fluorescence at the nanointerface, which provides high signal-to-noise ratio images of focal adhesion at the cell-attached interface. The advantage of this LSPR-assisted technique is its usability, which provides comparable or higher-quality nanointerfacial images than TIRF microscopy, even under epifluorescence microscopy. We also report the cytotoxicity of silver nanoparticles, as determined via morphological analysis of adherent cells on the sheet.
Highlights
The demand for super-resolution fluorescence microscopy is increasing within the field of cell biology because of the requirement to investigate molecular-level dynamic reactions in or near cells[1,2,3]
The optical microscopic images of the RBL-2H3 cells attached to oleylamine-capped gold nanoparticles (AuOA) after overnight incubation is shown for comparison in the supporting data
We reported that the optical field intensity excited by localized surface plasmon resonance (LSPR) on the AgMy sheet was confined to less than a 10 nm region from the centre of the particles, based on the finite-difference time-domain (FDTD) calculation[8]
Summary
The demand for super-resolution fluorescence microscopy is increasing within the field of cell biology because of the requirement to investigate molecular-level dynamic reactions in or near cells[1,2,3]. Is confocal laser scanning microscopy (CLSM) utilized as a standard tool for high-resolution fluorescence imaging, simulated emission depletion (STED) microscopy, structured illumination microscopy (SIM), photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM) are utilized as a family of super-resolution microscopy techniques. These super-resolution microscopy techniques have an advantage in their lateral resolution but are not as advantageous in either their axial resolution or temporal resolution because of their scanning criteria[4].
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