Abstract
Accurate cholesterol level measurement plays an important role in the diagnosis of severe diseases such as cardiovascular diseases, hypertension, anemia, myxedemia, hyperthyroidism, coronary artery illness. Traditionally, electrochemical sensors have been employed to detect the cholesterol level. However, these sensors have limitations in terms of sensitivity and selectivity. In this paper, a localized surface plasmon resonance (LSPR) -based biosensor is demonstrated that accurately detects and measures the concentration of cholesterol. In the present study, a tapered optical fiber-based sensor probe is developed using gold nanoparticles (AuNPs) and cholesterol oxidase (ChOx) to increase the sensitivity and selectivity of the sensor. Synthesized AuNPs were characterized by UV-visible spectrophotometer, transmission electron microscope (TEM), and energy dispersive X-ray spectroscopy (EDS). Further, coating of AuNPs over fiber was confirmed by scanning electron microscope (SEM). The developed sensor demonstrates for a clinically important cholesterol range of 0 to 10 mM, and the limit of detection is found to be 53.1 nM.
Highlights
Cholesterol is an essential component of human body
5.1 Reflection intensity before and after AuNPs-immobilization Reflection spectra were measured through bare taper fiber and AuNPs-immobilized taper fiber using experimental setup shown in Fig. 5. 10 mM of cholesterol solution was used to check the sensor probes
The sensor probe is functionalized with cholesterol oxidase (ChOx) that improves the selectivity of sensor
Summary
Cholesterol is an essential component of human body. It is produced by liver and is a part of healthy diet because it is precursor of very essential biological materials such as bile acid, steroid hormones [1] and vitamin D [2]. A low level of cholesterol causes various diseases such as anemia, hyperthyroidism, and malabsorption [6] Various conventional techniques such as gas chromatography (GC) [7], high-performance liquid chromatography (HPLC) [8], electrochemical methods [9], voltammetric [10], colorimetric [11] and non-enzymatic technique [12] are used for detection of cholesterol. These techniques are prone to several limitations such as low sensitivity, poor selectivity, complex and expensive development procedure. A fluorescence-based fiber optic biosensor using hydrogel-immobilized enzyme for cholesterol and glucose sensing has been proposed by Lin et al [21] but is temperature dependent
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
