LPS-induced locomotor and inflammatory responding in the stress sensitive WKY rat

Abstract

Event Abstract Back to Event LPS-induced locomotor and inflammatory responding in the stress sensitive WKY rat Marykate Killilea1*, Lisa Flannery1, Daniel M. Kerr2, Beth Mallard1, Antony Wheatley1 and Michelle Roche1 1 National University of Ireland, Galway, Physiology, Ireland 2 National University of Ireland, Galway, Pharmacology and Therapeutics, Ireland Recent data has demonstrated altered immune function including enhanced expression of toll-like receptors (TLRs), in the stress-hypersensitive Wistar Kyoto (WKY) rat. This study examined the effect of acute administration of the bacterial endotoxin and TLR4 agonist lipopolysaccharide (LPS) on locomotor activity and pro-inflammatory cytokine levels in WKY rats in comparison to the less stress-sensitive Sprague-Dawley (SD) strain. Home-cage locomotor activity monitoring revealed that male WKY rats exhibit reduced locomotor activity when compared to SD counterparts. Systemic administration of LPS (1 or 5mg/kg) or saline increased locomotor activity of all animals in the 30 minute time period post injection, an effect significantly blunted in WKY rats. Locomotor activity of SD LPS-treated rats was significantly reduced in between 90-150 minutes post administration when compared to saline-treated counterparts, an effect not observed in WKY rats. WKY rats exhibits lower liver and spleen to body weight ratio when compared to SD counterparts, an effect not altered by LPS. Assessment of pro-inflammatory cytokine levels revealed the LPS did not alter the levels of TNF-α, IL-6 or IL-1β in serum but significantly increased IL-1β, levels in liver of both SD and WKY when compared with saline-treated counterparts. Taken together, WKY and SD rats exhibit similar peripheral pro-inflammatory, but not locomotor, responses to TLR4 activation. Thus, neuro-immune signalling may underlie the altered behavioural response to TLR4 activation in this stress sensitive model. Acknowledgements Acknowledgements: Work supported by College of Medicine, postgraduate fellowship and Discipline of Physiology, NUI Galway. Keywords: LPS, Inflammation, stress sensitive WKY, locomotor activity, TLR4 Conference: Neuroscience Ireland Young Neuroscientists Symposium 2014 , Dublin, Ireland, 20 Sep - 20 Sep, 2014. Presentation Type: Poster Presentation Topic: Early Career Neuroscience Citation: Killilea M, Flannery L, Kerr DM, Mallard B, Wheatley A and Roche M (2014). LPS-induced locomotor and inflammatory responding in the stress sensitive WKY rat. Front. Neurosci. Conference Abstract: Neuroscience Ireland Young Neuroscientists Symposium 2014 . doi: 10.3389/conf.fnins.2014.87.00026 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 15 Sep 2014; Published Online: 15 Sep 2014. * Correspondence: Miss. Marykate Killilea, National University of Ireland, Galway, Physiology, Galway, Ireland, m.killilea2@nuigalway.ie Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Marykate Killilea Lisa Flannery Daniel M Kerr Beth Mallard Antony Wheatley Michelle Roche Google Marykate Killilea Lisa Flannery Daniel M Kerr Beth Mallard Antony Wheatley Michelle Roche Google Scholar Marykate Killilea Lisa Flannery Daniel M Kerr Beth Mallard Antony Wheatley Michelle Roche PubMed Marykate Killilea Lisa Flannery Daniel M Kerr Beth Mallard Antony Wheatley Michelle Roche Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.