LPS로 활성화된 RAW 264.7 대식세포에서 애기땅빈대(Euphorbia supina Rafin)의 염증매개물질 억제효과

Abstract

This study was designed to evaluate the effect of hot water extract (ESW) and 70% ethanol extract (ESE) from Euphorbia supina Rafin on LPS-stimulated inflammatory response in RAW 264.7 macrophages. Upon investigation at concentrations up to 1000 μg/mL, ESW and ESE did not have any cytotoxic effects on RAW 264.7 macrophages. ESW induced inhibition of 21.6%～54.8% of nitric oxide (NO) production at 100～1000 μg/mL, and PGE₂ production was inhibited up to 25.7%～38.2% at 250～1000 μg/mL, proportional to the ESW concentrations. ESW induced inhibition of 66.1% and 54.3% of IL-6 production at 250 and 1000 μg/mL, respectively. ESE (100～1000 μg/mL) induced inhibition of 38.3%～77.5% of NO, 40%～94.7% of PGE₂, and 43.9%～89.4% of IL-6 production, proportional to the ESE concentrations. Only 44.1% of IL-10 production was inhibited at a concentration of 500 μg/mL. ESE induced an increase in TNF-α production at a concentration of 100 and 250 μg/mL, whereas at high concentrations (500 and 1000 μg/mL), ESE induced inhibition of 19.2% and 92.4% of TNF-α production, respectively. In conclusion, concentrations of more than 500 μg/mL ESE demonstrated effective immune-modulating activity through inhibition of NO, PGE₂, IL-6, IL-10, or TNF-α production, as it relates to the macrophage's immuno-activity; therefore, ESE has potential as a good candidate substance for reduction of inflammatory responses.