LPS 주사한 BALB/c 마우스에서 Genistein의 산화적 스트레스 억제효과 및 항염증 효과

Abstract

School of Culinary Art & Baking Technology, Dong-Ju College, Busan 604-080, KoreaAbstractThis study was carried out to investigate the anti-oxidative and anti-inflammatory actions of genistein in BALB/c mice injected with lopopolysaccharide (LPS), called endotoxin. Mice (10 weeks of age) weighing approximately 20 g were divided into 4 groups. Endotoxin shock was induced by intraperitoneal injection of LPS (100 mg/kg BW). LPS and genistein＋LPS groups were injected with LPS 30 min after phosphate buffered saline (PBS) solution and genistein (200 mg/kg BW) injections, respectively. Genistein group was injected with genistein, followed by PBS, while PBS group received two injections of PBS. Superoxide anion generation of peritoneal macrophage cells was significantly (p<0.05) lower in the genistein＋LPS group than in the LPS injection group at 8 h after intraperitoneal injection, while SOD activity was significantly higher in genistien＋LPS group than LPS group. Tumor necrosis factor-α levels of plasma were significant lower (p<0.05) in the genistein＋LPS injection group than LPS group at 8 h after intraperitoneal injection. Plasma TBARS was lower in genistein＋LPS group than LPS group, while hepatic TBARS were not different among groups. Hepatic glutathione concentrations and antioxidant enzyme activities were significantly higher in the genistein＋LPS group than in the LPS group at 1 h and 8 h after intraperitoneal injection. Nuclear factor-kappa B (NF-κB) transactivation was significantly (p<0.05) inhibited in LPS group. These results demonstrate genistein may ameliorate inflammatory diseases through inhibition of NF-κB transactivation and oxidative stress, which may be mediated partially by anti-oxidative effect of genistein.Key words: anti-oxidative and anti-inflammatory effects, LPS, genistein, NF-κB, BALB/C mice