Abstract
In Vitro Anti-inflammatory Effects of Hyunbulikyung-tang Aqueous Extracts on LPS activated Raw 264.7 Cells, and Synergic Effects with Piroxicam Jung-Ah Kim, Dong-Chul Kim Dept. of Korean Obstetric & Gynecology, College of Korean Medicine, Deagu Haany University Objectives: To observe the in vitro anti-inflammatory effects of Hyunbulikyung-tang aqueous extracts (HBLKT) and the possible synergic combination effects with a nonsteroidal anti-inflammatory drug, piroxicam. Methods: Anti-inflammatory effects of HBLKT (yield=16.17%) were observed on LPS activated raw 264.7 cells based on ED50 to cell viability, NO, PGE2, TNF-α, IL-1β and IL-6 productions as compared with piroxicam, in the present study. In addition, the combination effects of HBLKT with piroxicam were observed after treatment of HBLKT 1/4 ED50 + piroxicam 1/4 ED50, 1/8 ED50, 1/16 ED50, 1/32 ED50 and 1/64 ED50 concentrations, respectively. Results: Significant (p<0.01 or p<0.05) increases of cell viabilities and decreases of NO, PGE2, TNF-α, IL-1β and IL-6 cytokine releases were detected in HBLKT 1/4 ED50 + piroxicam 1/4 ED50, 1/8 ED50, 1/16 ED50 and 1/32 ED50 concentration co-treatment as compared with each of single 1/4 ED50 concentration of piroxicam and HBLKT treatments, respectively. Although significant (p<0.01 or p<0.05) increases of cell viabilities and decreases of NO, PGE2, TNF-α, IL-1β and IL-6 cytokine releases were also demonstrated in piroxicam 1/64 ED50 + HBLKT 1/4 ED50 co-treatment as compared with LPS control, no significant changes were detected as compared with each of single 1/4 ED50 concentration of piroxicam and HBLKT treatments, in this experiment. Conclusions: Hyunbulikyung-tang showed cell protective and anti-inflammatory effects against LPS activated raw 264.7 cells. It, therefore, expected that HBLKT will be showed favorable effects to relieve dysmenorrhea related to over expressed inflammatory cytokines, and it also expected that the clinical dosages of piroxicam can be reduced as 1/8 levels as combination with HBLKT.
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