L-Phenylalanine catabolism and l-phenyllactic acid production by a phototrophic bacterium, Rubrivivax benzoatilyticus JA2

Abstract

A phototrophic bacterium (Rubrivivax benzoatilyticus JA2) grows at the expense of l-phenylalanine as sole source of nitrogen but not as carbon source. Near stoichiometric yields of l-phenylpyruvic acid (0.4mM) and l-phenyllactate (0.4mM) were observed from l-phenylalanine (0.9mM consumed). Aminotransfarase and dehydrogenase activities involved in the formation of l-phenylpyruvic acid and l-phenyllactate were demonstrated unequivocally in Rubrivivax benzoatilyticus JA2. Growth conditions and carbon sources had an influence on l-phenyllactate production. The process yielded a maximum of 0.92mM l-phenyllactate from l-phenylalanine (1mM) when fructose served as carbon source for R. benzoatilyticus JA2.