Abstract
A NMR method has been evaluated for the investigation of thermal and acid denaturation of transferrins in aqueous solutions. Heating an apoovotransferrin or an apolactoferrin solution resulted in a slight increase in T 1 -1 and an important increase in T 2 -1 relaxation rates within the denaturation range. It is proposed that the changes in NMR relaxation parameters can be used to monitor thermal denaturation of transferrins.
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