Lowering effects of aspirin eugenol ester on blood lipids in rats with high fat diet.

Isam Karam,Ning Ma,Jian-Yong Li,Xiao-Jun Kong,Ya-Jun Yang,Xiao-Le Zhao,Xi-Wang Liu

doi:10.1186/s12944-016-0369-2

Isam Karam, Ning Ma + Show 5 more

Open Access

https://doi.org/10.1186/s12944-016-0369-2

Copy DOI

Abstract

BackgroundAspirin and eugenol were esterified to synthesize aspirin eugenol ester (AEE). As a pale yellow and odourless crystal, AEE reduced the gastrointestinal damage of aspirin and vulnerability of eugenol. The study was conducted to evaluate the preventive effects of AEE on blood lipids in rats with high fat diet (HFD).MethodsSuspensions of AEE and simvastatin were prepared in 5% carboxymethyl cellulose sodium (CMC-Na). In order to observe the intervention effects, the drugs and HFD were administrated at the same time. Based on individual weekly body weight (BW), AEE was intragastrically administrated at the dosage of 18, 36 and 54 mg/kg. Simvastatin (10 mg/kg) and CMC-Na (20 mg/kg) were used as control drug. After 6 weeks of administration, the changes of BW and blood lipid indices including triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL) and total cholesterol (TCH) were determined in the experiment.ResultsThe rat blood lipids profile in model group was remarkably different after feeding 6-weeks HFD. TG, TCH and LDL indexes in model group were increased significantly compared with those in control group (p < 0.01). AEE at the dosage of 54 mg/kg significantly decreased levels of TG, TCH and LDL (p < 0.01), and slowed the rate of BW gain in comparison with model group (p < 0.05). Moreover, high dose AEE showed better effects than simvastatin on reducing TCH level and similar effects on TG, HDL and LDL.ConclusionAEE could remarkably reduce levels of TG, TCH and LDL in rats with high fat diet, and slow the rate of body weight gain. It was conducted that AEE was a potential candidate on reducing blood lipids level. The mechanism of action of AEE should be investigated in further studies.

Highlights

Aspirin and eugenol were esterified to synthesize aspirin eugenol ester (AEE)
Hyperlipidemia is a heterogeneous group of disorders characterized by an excess of lipids in blood stream such as the increased serum levels of triglycerides (TG), total cholesterol (TCH), low-density lipoprotein (LDL) as well as decreased levels of high-density lipoprotein (HDL) [1, 2]
Intervention effects of AEE After feeding rats with high fat diet (HFD) for 6 weeks, the blood lipid profile was notably different among the groups

Summary

Introduction

Aspirin and eugenol were esterified to synthesize aspirin eugenol ester (AEE). As a pale yellow and odourless crystal, AEE reduced the gastrointestinal damage of aspirin and vulnerability of eugenol. The study was conducted to evaluate the preventive effects of AEE on blood lipids in rats with high fat diet (HFD). Aspirin could ameliorate hyperlipidemia induced by high fat diet and hyperinsulinemia in rats [4]. Based on prodrug principle and therapeutic effects of aspirin and eugenol on hyperlipidemia, aspirin eugenol ester (AEE) as a new drug was synthesized [9]. AEE could be metabolized into aspirin and eugenol in vitro and in vivo, which could show their original activities and act synergistically [10]. AEE reduced the side effects of its precursor such as the gastrointestinal damage of aspirin and irritation of eugenol [11].

Methods

Results

Discussion

Conclusion