Lower HOMA-β values are detected among individuals with variant of E23K polymorphism of potassium inwardly-rectifying channel, subfamily J, member 11 (KCNJ11) gene

Abstract

BackgroundType 2 Diabetes Mellitus (T2DM) is a multifactorial disease involving both genetic and also environmental factors. Potassium inwardly-rectifying channel, subfamily J, member 11 (KCNJ11) gene, an ATP-sensitive potassium channel-coding gene, contributes to insulin secretion. ObjectivesThis research aimed to investigate E23K polymorphism in KCNJ11 gene and insulin secretion in individuals with family history of T2DM (cases) and without family history of T2DM (controls). MethodThis research was a case-control study involving 34 cases and 34 controls. E23K polymorphism of KCNJ11 was detected with PCR-RFLP. All of the obtained data were statistically analyzed with T-test, Mann–Whitney U-test, Chi-Square and One-Way ANOVA. ResultFrequency of AA genotype in individuals with family history of T2DM (41%) was higher than in individuals without family history of T2DM (6%) (p=0.001). Frequency of A allele in individuals with family history of T2DM (68%) was higher than in individuals without family history of T2DM (38%) (p=0.001). The risk of A allele in individuals with family history of T2DM was 3 times higher than in individuals without family history of T2DM (p=0.001, OR 3.38, CI 95% 1.67–6.84). Homeostasis Model Assessment β (HOMA-β) values of AA genotype (85.44%±39.55) were lower than that of GA (212.20%±79.30) and GG (254.00%±61.98) genotypes (p=0.000). ConclusionThe risk of having A allele in individuals with family history of T2DM is higher than that in individuals without family history of T2DM. HOMA-β values of AA genotype are lower than that of GA and GG genotypes.