Abstract
BackgroundsIntestinal alkaline phosphatase (IAP) plays important role in gut homeostasis. We aimed to evaluate the expression of endogenous IAP and to assess the clinical course according to the expression of endogenous IAP in patients with Crohn’s disease (CD).MethodsA total of 32 consecutive patients (14 males) with CD were included in the study. We measured the level of endogenous iAP in inflamed and noninflamed colonic mucosa. To verify the inflammation status, we measured the level of mRNA for IL-6, TNF-α, and TLR-4. We monitored the clinical courses of patients during follow-up after acquisition of biopsy specimens.ResultsMedian age of patients was 22.5 years (range, 15–49). Median CD activity index (CDAI, range) was 93.7 (22.8~ 154.9). There were colonic involvements in all patients and perianal involvement in 43.8% patients. The mRNA levels of IL-6 (p = 0.005) and TLR-4 (p = 0.013) in inflamed mucosa were significantly higher than those in non-inflamed mucosa. However, there was no difference of expression of TNF-α mRNA (p = 0.345). During a 14-month follow-up (range, 9 months–54 months), there were 19 patients with clinical recurrences. There were 9 patients (9/19, 47.4%) with IAP expression ratio (inflamed to non-inflamed) ≤ 1.0 in patients with clinical recurrence while there was one patient (1/13, 7.7%) with IAP ratio ≤ 1.0 in patients without clinical recurrence (p = 0.024).ConclusionLower expression of IAP in inflamed mucosa compared to non-inflamed mucosa may be associated with clinical recurrence in patients with CD.
Highlights
Intestinal alkaline phosphatase (IAP) is expressed by brush border of intestinal epithelium [1]
Demographics and baseline clinical characteristics A total of 32 consecutive patients (22 males) with Crohn’s disease (CD) were in clinical remission when they were included in the study
Expression of pro-inflammatory genes between the noninflamed mucosa and inflamed mucosa in patients with Crohn’s disease To verify the degree of inflammation between the inflamed and non-inflamed mucosa, we evaluated the mRNA levels of inflammatory genes
Summary
Intestinal alkaline phosphatase (IAP) is expressed by brush border of intestinal epithelium [1]. It plays an important role in the interaction between the gut flora and the host by detoxification of bacterial endotoxin, regulation of intestinal microbiome, and regulation of intestinal lipid absorption [2]. Low expression of IAP could increase the. Park et al BMC Gastroenterology (2018) 18:188 aimed to evaluate the clinical recurrences according to expression of endogenous IAP in colonic mucosa from patients with CD
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