Low water conductivity increases the effects of copper on the serum parameters in fish (Oreochromis niloticus)

Abstract

The conductivity is largely determined by ion levels in water, predominant ion being Ca2+ in the freshwaters. For this reason, the effects of copper were evaluated as a matter of conductivity of exposure media in the present study. Thus, freshwater fish Oreochromis niloticus were exposed to copper in differing conductivities (77, 163 and 330μS/cm), using acute (0.3μM, 3 d) and chronic (0.03μM, 30 d) exposure protocols. Following the exposure serum parameters of fish were measured.Data showed that there was no significant alteration (P>0.05) in serum parameters of control fish. However, activities of ALP, ALT and AST decreased significantly at the lower conductivities in chronic copper exposure, but not in acute ones. Protein levels did not differ significantly in any of the exposure conditions. However, Cu exposure at the lowest conductivity sharply increased the levels of glucose in the acute exposure, while there was no significant difference in the chronic exposure. Cholesterol levels decreased only at the lower conductivities in chronic exposure, but increased in acute exposure. Similarly, triglyceride levels increased in acute exposures and decreased in chronic exposures at the lowest conductivity. There was no change in Na+ levels, while there was an increase in K+ levels and a decrease in Ca2+ level at the lowest conductivity of acute exposures. However, Cl− levels generally decreased at the higher conductivities of chronic exposures. There was a strong negative relationship between significant altered serum parameters and water conductivity. In conclusion, this study showed that copper exposure of fish at lower conductivities caused more toxicities, indicating the protective effect of calcium ions against copper toxicity. Data suggest that conductivity of water may be used in the evaluation of metal data from different waters with different chemical characteristics.