Low volume PCR (LV-PCR) for STR typing of forensic casework samples

Abstract

In this study 1 μL low volume PCRs (LV-PCR) using a commercially available chip in microscopic slide format have been performed to test a wide range of forensic casework samples besides sensitivity and mixtures with different STR kits. Moreover critical low copy number and mixed DNA samples were dispensed into 20 individual LV-PCR assays to obtain a combined DNA profile.