Low temperature X-ray microanalysis of calcium in a scleractinian coral: evidence of active transport mechanisms.

Abstract

Element concentrations were measured by X-ray microanalysis in seawater (SW) compartments and mucocytes in bulk, frozen-hydrated preparations of the scleractinian coral Galaxea fascicularis. Quantitative X-ray microanalysis of polyps sampled in the daytime revealed that concentrations of the elements Na, S, K and Ca were all significantly higher in a thin (10-20 micro m) external SW layer adjacent to the oral ectoderm (P<0.05, <0.05, <0.0001 and <0.01, respectively) than in standard SW. In polyps sampled during night-time, concentrations of Ca and S in this external SW layer were significantly reduced (P<0.05). Ca concentration in the coelenteron and extrathecal coelenteron was significantly higher (P<0.001) than in the external SW layer, regardless of time of sampling, suggesting that Ca(2+) transport across the oral epithelium occurs via an active, transcellular route. X-ray microanalyses of mucocytes revealed that the concentration of S was high and did not vary between epithelial layers, while that of Ca increased in an inward gradient toward the skeleton. We suggest that throughout the day, secreted mucus behaves as a Donnan matrix at the oral ectoderm-SW interface, facilitating intracellular Ca(2+) uptake. The accumulation within internal SW compartments of high concentrations of Ca relative to standard SW levels, however, appears to be independent of mucus secretion and is likely to be a consequence of active transport processes.