Low temperature triggers physiological and behavioral shifts in adult oriental armyworm, Mythimna separata

Abstract

Migratory insects display diverse behavioral strategies in response to external environmental shifts, via energy allocation of migration-reproduction trade-offs. However, how migratory insects distribute energy between migration and reproduction as an adaptive strategy to confront temporary low temperatures remains unclear. Here, we used Mythimna separata, a migratory cereal crop pest, to explore the effects of low temperature on reproductive performance, behavior, and energy allocation. We found that the influence of low temperatures on reproduction was not absolutely negative, but instead depended on the intensity, duration, and age of exposure to low temperature. Exposure to 6°C for 24 h significantly accelerated the onset of oviposition and ovarian development, and increased the synchrony of egg-laying and lifetime fecundity in 1-day-old adults compared to the control, while female's flight capacity decreased significantly on the first and second day after moths were exposed to 6°C. Furthermore, the abdominal and total triglycerides levels of females decreased significantly from exposure to low temperature, but their thoracic triglyceride content was significantly higher than the control on the third and fourth day. These results indicated that low temperatures induced M. separata to reduce energy investment for the development of flight system. This resulted in the shifting of moths from being migrants to residents during the environmental sensitive period (first day post-emergence). This expands our understanding of the adaptive strategy employed by migratory insects to deal with low temperatures and aids in the management of this pest species in China.