Abstract
A standard low temperature steam/formaldehyde autoclave was tested according to the manufacturer's instructions, using a range of test pieces containing Bacillus stearothermophilus spores as the challenge organism. There were failures in killing the challenge organism and the reasons for these are discussed. A description of modifications made to the autoclave is given together with details of an improved operating cycle. The performance of the modified autoclave was greatly improved and conditions were established for reliable and consistent sterilization of all the test pieces. A commercially produced prototype autoclave similarly modified also showed effective sterilization.
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