Abstract
Cold acclimation is the development of increased freezing tolerance in competent genotypes upon exposure to low but non-freezing temperatures over a period of days or weeks (Levitt, 1980). In nature, it is triggerred by declining temperature during the onset of winter and is quickly lost as temperature rises during spring. Cold acclimation can be reproduced in the laboratory by exposing the seedlings or cell suspension cultures to 2–5°C for a prolonged period and rapid deacclimation can be observed on returning the samples to 25°C.
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