Year-end Sale % OFF 
Abstract
Antimicrobial resistance is a steadily increasing problem and poses a serious threat to global public health. Therefore, it is highly necessary to advance the development of novel antimicrobial compounds and semen preservation strategies. The aim of this study was to evaluate a low temperature, antibiotic-free preservation procedure using Androstar Premium (ASP) extender (Minitüb) with antimicrobial lipopeptides. Firstly, seven lipopeptides in two concentrations (1 × minimum inhibitory concentration (MIC)/2 × MIC) were tested on their sperm-compatibility at 17 °C. Two lipopeptides, C16-KKK-NH2 and C16-KKKK-NH2, did not negatively affect sperm quality and were further evaluated for their efficiency of bacterial growth inhibition at 5 °C. Besides an overall diminution of colony forming units, both peptides showed a reduction of bacterial subcultures (n = 103) with a decrement in Gram-positive rods from 65 (ASP w/o supplements) to 39/52 (ASP w/ C16-KKK-NH2/C16-KKKK-NH2), in Gram-positive cocci from 21 to 9/10 and in Gram-negative species from 17 to 8/5 total subcultures. Furthermore, lipopeptides revealed activity towards selected bacteria of potential concern in artificial insemination like Trueperella pyogenes, Alcaligenes faecalis, Pseudomonas aeruginosa (not C16-KKK-NH2), Pasteurella sp., Providencia stuartii, Escherichia coli (not C16-KKKK-NH2) and Streptococcus porcinus (not C16-KKKK-NH2). Consequently, both tested lipopeptides are promising candidates for alternative antibiotic-free preservation techniques of boar semen.
Highlights
Antimicrobial resistance is a steadily increasing problem and poses a serious threat to global public health
After testing the different lipopeptides on their influence on sperm quality, the combination of a cold-temperature storage at 5 °C with the addition of the most spermcompatible lipopeptides was examined on synergistic effects concerning the inhibition of bacterial growth and the preservation of a sufficient sperm quality in order to find possible alternatives for conventional antibiotics used in the pig artificial insemination (AI) industry
This became apparent in the following cases: C14-KKKK-NH2 had no effect on TRT30 and (C10)2-KKKK-NH2 did not reduce mitochondria activity (MITO) or TRT30
Summary
Antimicrobial resistance is a steadily increasing problem and poses a serious threat to global public health. Even though there have been promising results regarding the use of AMPs in AI in pigs, there are many challenges, like the expensive and often time-consuming synthesis, insufficient antimicrobial activity or detrimental effects on sperm quality[14] Another approach towards the waiving of antibiotics in the pig AI industry is the low temperature storage of extended boar spermatozoa, which helps to minimize bacterial g rowth[15] and could help to reduce the development of multi-resistant bacteria with absent selection pressure of antibiotics. After testing the different lipopeptides on their influence on sperm quality, the combination of a cold-temperature storage at 5 °C with the addition of the most spermcompatible lipopeptides was examined on synergistic effects concerning the inhibition of bacterial growth and the preservation of a sufficient sperm quality in order to find possible alternatives for conventional antibiotics used in the pig AI industry
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
