Abstract
The potential applications of low temperature plasma (LTP) in wound healing have aroused the concern of many researchers. In this study, an argon atmospheric pressure plasma jet was applied to generate LTP for treatment of murine fibroblast cell (L929) cultured in vitro to investigate the effect of NF-κB pathway on fibroblast proliferation. The results showed that, compared with the control, L929 cells treated with plasma for less than 20 s had significant increases of proliferation; the productions of intracellular ROS, O2− and NO increased with prolongation of LTP treatment time; NF-κB pathway was activated by LTP in a proper dose range, and the expression of cyclinD1 in LTP-treated cells increased with the same trend as cell proliferation. After RNA interference to block p65 expression, with the same treatment time, RNAi-treated cells proliferated more slowly and expressed less cyclinD1 than normal cells. Furthermore, pretreatment with N-acetyl-L-cysteine (NAC) markedly prevented the plasma-induced changes in cells. In conclusion, the proliferation of L929 cells induced by LTP was closely related to NF-κB signaling pathway, which might be activated by appropriate level of intracellular ROS. These novel findings can provide some theoretical reference of LTP inducing cell proliferation and promoting wound healing.
Highlights
Low temperature plasma (LTP) has been used for sterilization in biomedical fields for several years[1,2,3]
Through analyzing the expression of cyclinD1 extracted from low temperature plasma (LTP)-treated cells, we discovered that the changes of cyclinD1 expression had the same trend with cell proliferation
The emission spectra of the plasma jet were collected by a spectrometer (Mechelle 5000, Andor Technology Ltd., Belfast, UK) together with an intensified charge coupled device (ICCD)
Summary
Low temperature plasma (LTP) has been used for sterilization in biomedical fields for several years[1,2,3]. LTP is composed of complex chemical compositions, such as exited atoms, electrons, ions, free radical, UV, and so on[16] These active particles can react with cell culture medium and tissues to form reactive oxygen and nitrogen species (RONS). We presume that LTP can induce L929 cell proliferation by activating NF-κB signaling pathway. When the NF-κB pathway was blocked with RNA interference, RNAi-treated cells proliferated more slowly and expressed less cyclinD1 than normal cells with the same treatment time. These findings will provide some beneficial support of LTP inducing cell proliferation and promoting wound healing
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