Abstract
Peel tissue from deacclimated late season grapefruit was examined for the capacity to develop cold induced acid invertase. Experimental fruit were subjected to three prolonged temperature treatments (17 days at 25°C, or 17 days at 4°C ± an additional 24 h at 25°C) and the flavedo examined for acid invertase activity immediately after the respective temperature treatment. Invertase activity was not detected when soluble extracts were assayed directly after desalting. However, after isoelectric separation, invertase activity was detected only in samples from cold treated fruit. Cell-wall pellets were also devoid of invertase activity when measured directly. After washing with high ionic buffer (containing 1 M NaCl), activity was recovered in the same cold treated samples. The data indicate that the peel of late season grapefruit retain the capacity to develop acid invertase in response to cold treatment.
Published Version
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