Low Temperature Induced Confined Effect for Aerolysin Nanopore Sensing

Abstract

Wildtype aerolysin nanopore sensing shows a high resolution for short oligonucleotides, which directly identifies single-nucleotide difference in both length and base. This sensibility mainly results from two sensitive regions. Therefore, previous designs of mutant aerolysin nanopore prolongs the duration time of oligonucleotide to achieve an improved accuracy for nucleotide identification. In order to further understand sensing mechanism, herein, the aerolysin nanopore sensing was carried out using poly(dA)4 as an analyte probe and at a temperature below 0 °C.