Abstract
The relaxation rate of primary excited native (124kDa) phytochrome from oat, is measured by time-resolved fluorescence techniques at temperatures between 77 and 300 K in a buffer/glycerol mixture. The known multiexponential fluorescence decay of the red absorbing form P r is found at physiological temperatures while it can be characterized by a single exponential below 160 K with a lifetime of (1.3 ± 0.2) ns at 77 K. This is consistent with the dynamics of the initially excited P r and an excited intermediate of the chromophore during the Z-E photoisomerization.
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