Abstract
The aim of this investigation is preparation of Mitomycin-C encapsulated with chitosan nanoparticles synthesis using ionic gelation technique for intravesical controlled drug delivery systems. This study was conducted in vitro. Cumulative amount of drug released from the nanoparticles was calculated. Mitomycin-C release studies were examined for different pH values. During the drug loading and release studies, initial amount of drug was changed (i.e., 0.5, 1.25 and 2.5 mg) to get different release profiles and the release studies were repeated (n = 6). The loading efficiencies of Mitomycin-C with three different initial concentrations 0.5mg/ml, 1.25 mg/ml and 2.5 mg/ml into chitosan nanoparticles were 54.5%, 47.1% and 36.4%, respectively. For different pH values, the cumulative releases of Mitomycin-C from chitosan nanoparticles were 47% and 53% for pH 6.0 and 7.4, respectively (p p san nanoparticles was measured in T24 bladder cancer cell line in vitro, and the results revealed that the 2.5 MMC coated Chitosan nanoparticles had better tumor cells decline activity. From this investigation, we conclude that the drug encapsulated synthesized chitosan nanoparticles possess a high ability to be used as pH and dose responsive drug delivery system. This systematic investigation demonstrates a promising future for the intravesical installation in treatment of the superficial bladder cancer.
Highlights
Bladder carcinoma is one of the most predominant malignancies of the urinary tract
The anticancer activity of Mitomycin-C loaded chitosan nanoparticles was measured in T24 bladder cancer cell line in vitro, and the results revealed that the 2.5 MMC coated Chitosan nanoparticles had better tumor cells decline activity
Morphological evaluation showed that the obtained nanoparticles were sphere like in shape with a narrow size distribution range at around 140 nm as shown in dynamic light scattering output and the polydispersity (Q) was 0.370
Summary
Bladder carcinoma is one of the most predominant malignancies of the urinary tract. Most bladder tumors are superficial at the diagnostics stage, since the symptoms are nonspecific and may be linked with many other conditions. Many urothelial cell carcinomas diagnoses are not invasive, since they go no deeper than the superficial layer (mucosa) of the bladder [1]. These tumors can be treated by surgical resection and adjuvant intravesical installation of Bacille Calmette Guerine (BCG) and other chemotherapeutic agents including Mitomycin-C (MMC), Epirubicin, Doxorubicin and Adriamycin [2] [3] [4] [5]. Some methods such as microwave-induced hyperthermia or electromotive drug administration, have been known to increase efficacy of MMC, the optimal usage of MMC has not been described [9]. The optimal MMC molecule demonstrates certain characteristics, such as long-acting, reduced administration of repeated dose, reduced side-effect and low dose requirement, there is no known ideal MMC molecule
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