Low-power infrared laser modulates mRNA levels from genes of base excision repair and genomic stabilization in heart tissue from an experimental model of acute lung injury.

Abstract

The aim of this study was to evaluate photobiomodulation effects on mRNA relative levels from genes of base excision repair and genomic stabilization in heart tissue from an experimental model of acute lung injury by sepsis. For experimental procedure, animals were randomly assigned to six main groups: (1) control group was animals treated with intraperitoneal saline solution; (2) LASER-10 was animals treated with intraperitoneal saline solution and exposed to an infrared laser at 10Jcm-2; (3) LASER-20 was animals treated with intraperitoneal saline solution and exposed to an infrared laser at 20Jcm-2; (4) acute lung injury (ALI) was animals treated with intraperitoneal LPS (10mgkg-1); (5) ALI-LASER10 was animals treated with intraperitoneal LPS (10mgkg-1) and, after 4h, exposed to an infrared laser at 10Jcm-2 and (6) ALI-LASER20 was animals treated with intraperitoneal LPS (10mgkg-1) and, after 4h, exposed to an infrared laser at 20Jcm-2. Irradiation was performed only once and animal euthanasias for analysis of mRNA relative levels by RT-qPCR. Our results showed that there was a reduction of mRNA relative levels from ATM gene and an increase of mRNA relative levels from P53 gene in the heart of animals with ALI when compared to the control group. In addition, there was an increase of mRNA relative levels from OGG1 and APE1 gene in hearts from animals with ALI when compared to the control group. After irradiation, an increase of mRNA relative levels from ATM and OGG1 gene was observed at 20Jcm-2. In conclusion, low-power laser modulates the mRNA relative levels from genes of base excision repair and genomic stabilization in the experimental model of acute lung injury evaluated.