Abstract
Low molecular weight (LMW) fucoidan, obtained by free radical depolymerization of high molecular polysaccharide extract of brown algae Hizikia fusiformis, was complexed with HPLC purified bee venom melittin. Water soluble form of the LMW fucoidan – melittin complex shows increased anti-inflammatory activity, inhibiting the production of nitric oxide in murine macrophage cell line Raw 264.7. The LMW fucoidan:melitin complex obtained in this study showed good biological activities, resulting in 2-fold reduction of the melittin toxicity. The fucoidan: melittin macromolecular complex obtained should be useful in future therapeutic applications.
Highlights
The clinical importance of many venomous compounds underlies the development of innovative approaches to minimize their cytotoxicity level during therapeutic applications in disease treatments [1]
The Low molecular weight (LMW) fucoidan: melitin complex obtained in this study showed good biological activities, resulting in 2-fold reduction of the melittin toxicity
It’s noteworthy to mention that the molecular masses of LMW fucoidan (3145) and melittin (2840) are comparable, which allows calculating a molar ratio from weight concentration
Summary
The clinical importance of many venomous compounds underlies the development of innovative approaches to minimize their cytotoxicity level during therapeutic applications in disease treatments [1]. In this context, a screening of efficient macromolecular complexes with therapeutic venoms, helping to reduce a generic toxicity, is one of the expanding research areas to exploit venomous molecules of animal and plant origin [1]. Molecular mass of fucoidan is heterogenic (10 - 1000 kDa) and difficult for making measurement standards in studying of its biological activity. There is no evidence of fucoidan’s toxicity in the literature and purified fucoidan is nontoxic in cell and animal test systems [8]
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