Abstract
Outcrossing rates were estimated in two artificial stands of Pinus leucodermis, a wind pollinated conifer characterized by a relatively high level of selfing. Multilocus outcrossing rates, estimated by assaying five enzyme loci, were lower than 86% in the two stands, using both dormant and germinating seeds, and not different from those observed in two natural populations located in the same area. The lack of relevant differences between single-locus minimum variance means and the multilocus outcrossing rate estimates observed in both the artificial stands indicates that most of the apparent selfing is probably caused by actual self-fertilization. The presence of a high level of self-fertilization in this species seems confirmed also in artificial stands, where the density is higher and neighbourhood structure should be less pronounced. The high level of self-fertilization found in P. leucodermis, which is probably due to the presence of a reduced number of recessive embryonic lethals as a consequence of genetic drift, could represent a selective advantage for this pioneering and precocius species. Key words: mating system, outcrossing rates, artificial stands, Pinus leucodermis.
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