Abstract
The genetic diversity of the turfgrass pathogen, Leptosphaeria korrae was examined with random amplified polymorphic DNA (RAPD) markers at the sibling, population, regional and cross-continental levels using 71 isolates. The seven RAPD primers tested showed polymorphisms within and between regions in North America. No polymorphisms were observed between sibling spores nor between twenty-one isolates from a single field. Based on differences observed with 48 polymorphic RAPD markers, only six unique but closely related haplotypes were identified among 33 geographically separated field isolates from British Columbia, Ontario, and Quebec in Canada, and Washington State in the USA. Although there was clustering of isolates of the same haplotype, some haplotypes had wide distributions across different regions. We hypothesize that few haplotypes of L. korrae were introduced into these regions from the centre of origin.
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