Abstract
Present study examined whether low‐level laser (LL) in combination with transplantation of mesenchymal stem cells (MSC) have synergistic effects leading to functional recovery that is greater than MSC therapy alone. Adult male SD rats received sciatic nerve crush injury by the vessel clamp for 20‐minute were divided into three groups: denervated + MSC transplant + LL (group I), denervated + MSC transplant (group II) and denervated (group III). MSC isolated from rat bone marrow was injected into the lesion site in group I and II and culture medium for group III. Three days after surgery, group I received GaAlAs LL irradiation (660nm, 9 J/cm2) for 7 days. Functional assessments including sciatic function index (SFI), vertical activity (VA), angle of ankle (AA), and electrophysiological studies were performed. Histological analysis was made to assess nerve continuity and myelination. Significant differences in SFI, VA, AA, amplitudes and onset latencies of compound muscle action potentials were found between group I and II. Decreased vacuole formation and axon degeneration examined by haematoxylin and eosin staining was found in group I. Results showed that the MSC/LL‐combined treatment had the most beneficial effect which may involve the attenuation of inflammatory responses by LL. This study was supported by a grant from the National Science Council (NSC 96‐2314‐B‐241‐001‐MY3) of Taiwan.
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