Abstract
Low intensity pulsed ultrasound (LIPUS) has been widely used in clinic for the treatment of repairing pseudarthrosis, bone fractures and of healing in various soft tissues. Some reports indicated that LIPUS accelerated peripheral nerve regeneration including Schwann cells (SCs) and injured nerves. But little is known about its appropriate intensities on autograft nerves. This study was to investigate which intensity of LIPUS improved the regeneration of gold standard postsurgical nerves in experimental rat model. Sprague-Dawley rats were made into 10 mm right side sciatic nerve reversed autologous nerve transplantation and randomly treated with 250 mW/cm2, 500 mW/cm2 or 750 mW/cm2 LIPUS for 2–12 weeks after operation. Functional and pathological results showed that LIPUS of 250 mW/cm2 significantly induced faster rate of axonal regeneration. This suggested that autograft nerve regeneration was improved.
Highlights
Nerve injury models used to explore the promoting regeneration effect on peripheral nerve were sciatic nerve crush model[17], sciatic nerve neurotomy model[13] and PLGA or silicon conduits interposed into sciatic nerve defect gaps model[18] in mammals
Previous LIPUS in vivo studies were in the peripheral nerve repairation after injuries, but few studies were in the nerve regeneration after the gold standard treatment-autologous nerve transplantation
LIPUS of 49.6 mW/cm[2] and 57 mW/cm[2] intensities, 1 MHz repetition pulse rate were initially used in the repairing of pseudarthrosis and bone fractures by Duarte in 198320
Summary
Nerve injury models used to explore the promoting regeneration effect on peripheral nerve were sciatic nerve crush model[17], sciatic nerve neurotomy model[13] and PLGA or silicon conduits interposed into sciatic nerve defect gaps model[18] in mammals. Previous LIPUS in vivo studies were in the peripheral nerve repairation after injuries, but few studies were in the nerve regeneration after the gold standard treatment-autologous nerve transplantation. The purpose of this study is to explore the therapeutic effect of three different ultrasound intensities (250 mW/cm[2, 500] mW/cm[2] and 750 mW/cm2) on the grafts of autologous nerve reversal transplantation. The evaluation of target organs was usually by comparing weighing muscles and the cross-sectional areas of fibers. We first apply contrast-enhanced ultrasonography (CEUS) for evaluating muscle functional recovery on the autograft animal models
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
