Low-intensity pulsed ultrasound modulates multi-frequency band phase synchronization between LFPs and EMG in mice

Abstract

Objective. Low-intensity pulsed ultrasound stimulation (LIPUS) targeted to the mouse motor cortex can simultaneously induce local field potentials (LFPs) and electromyogram (EMG) responses. However, the functional coupling relationship between LFP and EMG signals has not been elucidated to date. This study aimed to investigate the phase synchronization between LFP and EMG signals induced by LIPUS over the mouse motor cortex. Approach. LIPUS at 500 kHz with varied sonication intensities and duty cycles (DCs), was delivered to the mouse motor cortex. LFPs of the motor cortex and EMG responses of the tail were simultaneously recorded during LIPUS. We then evaluated two control groups using the same experimental parameters, but changed the position of EMG recording to the hind leg and the ultrasound stimulus target to the primary visual cortex. The phase synchronization between LFPs and EMG signals was evaluated by performing a phase locking value (PLV) analysis in the time-frequency domain and was compared across specific frequency bands. Main results. The results showed that LIPUS increased the phase synchronization in a broad frequency band (5–150 Hz), and the maximum duration of the increased PLV was stable at approximately 200 ms. It is worth noting that the sonication parameters directly affected the time-frequency domain distribution of cortico-muscular synchronization. Specifically, significant alpha and beta synchronization appeared at 0.2 and 0.4 W cm−2 Isppa stimulation, while gamma synchronization occurred at 0.8 and 1.1 W cm−2 Isppa stimulation. The synchronization in all frequency bands apparently increased at 30% DC. Beta synchronization weakened when the DC was less than 30%. Furthermore, no significant phase synchronization was observed in the two control groups. Significance. Considering the close association between specific motor function and cortical-muscular synchronization in different frequency bands, we suggest that LIPUS over the motor cortex could selectively modulate motor function via different sonication parameters. Additionally, the phase synchronization between LFPs and EMG might be an invaluable index for assessing the ultrasonic effect on the motor system, which could be used in future clinical research to optimize ultrasound parameters. Thus, this study provides new insight into evaluating the neuromodulatory effects of ultrasound on motor function, thereby supporting the therapeutic application of ultrasound in neurological disorders characterized by motor deficits.