Abstract
Glutamine (gln) is frequently added to equine semen as a diluent to improve spermatozoa life, however there is little information about concentration of glutamine metabolism in equine testis. Gln and Glu concentrations were determined, by enzymatic analyses, in regions near the testicular vein (TA), near the external area of the testis (TB), the head of the epididymus (EH), and the tail of epididymus (ET). Samples were obtained from stallions (n=10) that underwent surgery to remove the testes. ANOVA and Tukey's test were used analyze the results, with P<5%. Tissues were washed to remove the semen present before analysis. Results showed significant diference in [glu+gln] between all four tissues. Lower [glu+gln] was observed in in EH (5.781±1.073 umol/mg of tissue) when compared with other 3 areas (ET: 10.970 ± 0.782; TA: 13.272±0.671; TB:10.840 ±1.463) (P<0.05). Overall, this work indicates considerable differences in glutamine plus glutamate concentrations in different regions of the testis. However to determine more precise the glutamine metabolism in all four tissues we need to determine GS and glutaminase activity in these tissues, and a definitive interpretation can be presented.
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