Abstract
BackgroundSome studies suggest that Human Papilloma Virus (HPV) infection is important factor in carcinogenesis of breast tumors. This study’ objective was to analyze HPV prevalence in breast cancers of patients from south-central Poland.Materials and methodsThe study was performed based on archival paraffin embebbed and formalin fixed blocks in the group of 383 patients with breast cancer. HPV prevalence and its genotype were assessed, respectively by: nested PCR (with two groups of primers: PGMY09/PGMY11 and GP5+/GP6+), quantitative PCR (qPCR). Tumors were classified as HPV positive in case of at least one positive result in nested PCR and positive results in genotyping procedure. For all HPV positive tissues P16 immunostaining was applied in order to confirm active viral infection.ResultsIn the group of 383 breast cancers, HPV positivity was found in 17 samples (4.4%) in nested PCR. All these samples were subjected to HPV genotyping. This analysis revealed presence of HPV type 16 into two tumors (0.5%). In these two cancers, P16 overexpression was reported.ConclusionIn breast tumors of patients from south-central Poland in Poland, HPV positivity is demonstrated in very low percentage of cases.
Highlights
According to the analysis based on the GLOBOCAN database from 2018, 2.2 million infection-attributable cancers (13% of all cancer cases) were diagnosed [1]
In the group of 383 breast cancers, Human Papilloma Virus (HPV) positivity was found in 17 samples (4.4%) in nested PCR
In breast tumors of patients from south-central Poland in Poland, HPV positivity is demonstrated in very low percentage of cases
Summary
According to the analysis based on the GLOBOCAN database from 2018, 2.2 million infection-attributable cancers (13% of all cancer cases) were diagnosed [1]. 000 new diagnosed cancers [1] This infection (mainly HPV16), is an approved risk factor in development of some anogenital cancers (cervical, vulvar, vaginal, penile and anal cancers) and tumors localized in head and neck region. HR-HPV infection is proposed as a risk factor in development of breast cancers (BC). In a few meta-analyses [2,3,4,5,6], it was shown that exposure to HR-HPV is related to increase of relative risk of breast cancer development from 3.24 [4] to 5.9 [5]. Some studies suggest that Human Papilloma Virus (HPV) infection is important factor in carcinogenesis of breast tumors. This study’ objective was to analyze HPV prevalence in breast cancers of patients from south-central Poland
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