Abstract
BackgroundProtein arginine methyltransferases (PRMTs) can catalyse the methylation of arginine and participate in many important cellular reaction processes. The purpose of this research is to determine whether the expression levels of the PRMT5 gene in peripheral blood can be used as a biomarker for predicting the risk of Acute Myocardial Infarction (AMI).MethodsIn this research, peripheral blood was collected from 91 patients with AMI and 87 patients with stable coronary artery disease (CAD). Real-time fluorescent quantitative PCR was performed to measure the expression levels of the PRMT5 gene at the mRNA level, and a western blot analysis was performed to measure the expression levels of the PRMT5 gene at the protein level.ResultsThe results indicate that at both the RNA and protein levels, the expression levels of the PRMT5 gene in peripheral blood from patients with AMI are significantly lower than those in peripheral blood from patients with stable CAD (Z = − 4.813, P = 0.000). The low expression of the PRMT5 gene is relevant to the Gensini score of the coronary artery (rs = − 0.205, P = 0.015) but is irrelevant to the serum level of blood lipids, level of cardiac troponin (rs = − 0.125, P = 0.413) and time intervals of occurrence (rs = − 0.146, P = 0.211). Patients who have a low PRMT5 expression in the peripheral blood are 5.472 times more likely to suffer from AMI than other patients.ConclusionCompared to stable CAD patients, AMI patients have a lower expression of the PRMT5 gene in their peripheral blood. Patients who have low PRMT5 gene expression in the peripheral blood are more likely to suffer from AMI than those with stable CAD. A low expression of the PRMT5 gene serves as an independent risk factor for the occurrence of AMI.
Highlights
Protein arginine methyltransferases (PRMTs) can catalyse the methylation of arginine and participate in many important cellular reaction processes
Clinical data analysis The results of the clinical data analysis of the research objects indicate that there is no significant difference between the two groups in the aspects of age, sex, body mass index (BMI), history of hypertension, family history of coronary heart disease, systolic pressure, diastolic pressure, serum triglyceride (TG) and high-density lipoprotein cholesterol (HDL-C)
The results indicate that there is no difference in the expression level of the PRMT5 gene at the mRNA level between the normal total cholesterol (TC) level group and the increased TC level group (P = 0.363); there is no difference in the expression level of the PRMT5 gene at the mRNA level between the normal low-density lipoprotein cholesterol (LDL-C) level group and the increased LDL-C level group (P = 0.568); there is no difference in the expression level of the PRMT5 gene at the mRNA level between the ACEI/angiotensin receptor blocker (ARB) group and the ACEI/ARB group (P = 0.177)The expression level of the PRMT5 gene at the mRNA level is irrelevant to the EF value of the patients (P = 0.993)
Summary
Protein arginine methyltransferases (PRMTs) can catalyse the methylation of arginine and participate in many important cellular reaction processes. The purpose of this research is to determine whether the expression levels of the PRMT5 gene in peripheral blood can be used as a biomarker for predicting the risk of Acute Myocardial Infarction (AMI). Numerous studies have indicated that the gene expression levels in peripheral blood can reflect changes in and the progression of complex cardiovascular diseases, serving as a very significant biomarker for examining and diagnosing cardiovascular diseases [6, 7]. The expression level of single genes at the mRNA level in peripheral blood can be used as a genetic marker for the assessment of disease progression, and the overexpression of KIAA0101 at the mRNA level can be used as a predictor of the invasion and progression of liver cancer [10], while the expression of KLKB1 at the mRNA level in monocytes in peripheral blood can be used as a molecular marker for the diagnosis and identification of chronic lymphocytic leukaemia patients and non-leukaemia patients [11]. The expression level of CD36 at the mRNA level in monocytes in peripheral blood may mark the progression of coronary heart disease [12], and the expression level of AdipoR2 at the mRNA level in peripheral blood is correlated with the progression of coronary atherosclerosis [13]
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