Abstract
BackgroundThe long non-coding RNAs (lncRNAs) study has gradually become one of the hot topics in the field of RNA biology. One lncRNA which has attracted attention is LOC285194, a lncRNA demonstrated the potential tumor-suppressor role in osteosarcoma. The aim of this study was to examine the expression of LOC285194 in colorectal cancer (CRC) patients and to investigate the relationship between this lncRNA levels and existing clinicopathologic parameters and patient survival.MethodsThe expression of LOC285194 was detected by quantitative real-time polymerase chain reaction in pairs of tumorous and adjacent normal tissues of 81 colorectal cancer patients with a follow-up of 5 years, as well as in three colorectal cancer cell lines and normal intestinal mucous cell line. Then, we analyzed the potential relationship between this lncRNA levels in tumor tissues and existing clinicopathological features of CRC, and clinical outcome.ResultsThe relative expression levels of LOC285194 was significantly lower in tumor tissues (p < 0.001) and colorectal cancer cell lines compared with adjacent normal tissues and normal intestinal mucous cell line. In addition, low expression of LOC285194 was correlated with larger tumor size (p = 0.015), higher tumor stage (p = 0.034), and more distant metastasis (p = 0.046). Kaplan-Meier analysis indicated that patients with low LOC285194 expression had a poor disease free survival (p = 0.010). Moreover, multivariate analysis showed that decreased expression of LOC285194 was an independent predictor of disease-specific survival.ConclusionOur data indicate that LOC285194 might be a novel prognostic indicator in colorectal cancer and may be a potential target for diagnosis and gene therapy.
Highlights
IntroductionThe long non-coding RNAs (lncRNAs) study has gradually become one of the hot topics in the field of RNA biology
The long non-coding RNAs study has gradually become one of the hot topics in the field of RNA biology
Expression of LOC285194 in tissues and cell lines The first goal of the present study was to investigate whether LOC285194 is detectable and altered in colorectal cancer tissues compared with adjacent normal tissues
Summary
The long non-coding RNAs (lncRNAs) study has gradually become one of the hot topics in the field of RNA biology. The aim of this study was to examine the expression of LOC285194 in colorectal cancer (CRC) patients and to investigate the relationship between this lncRNA levels and existing clinicopathologic parameters and patient survival. For accurate diagnosis and adequate treatment of CRC, identification and Except for about 2% protein-coding genes, the vast majority of the human genomes are non-coding RNAs (ncRNAs) [2], implicating that ncRNAs could play significant regulatory roles in complex organisms. The function and clinical significance of short regulatory ncRNAs, such as microRNAs (miRNAs) and smallinterfering RNAs (siRNAs) were elucidated first and long ncRNAs (lncRNAs) were reported more recently. Recent reports showed that some lncRNAs exhibit distinct gene expression patterns in solid tumors and leukemias [4,5,6,7,8]. Functional lncRNAs can be used for cancer diagnosis and prognosis, and serve as potential therapeutic targets, we consider them as a new cancer diagnostic and therapeutic gold mine in the future [9]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
