Abstract
This report describes a direction-dependent optical microscope operated with Raspberry Pi, with LED array illumination patterns controlled by Raspberry Pi. Images are obtained with a Raspberry Pi camera module. We demonstrate the use of Raspberry-Pi-based microscopes for multimodal imaging including bright-field microscopy, dark-field microscopy, differential phase-contrast microscopy, and polarization microscopy. We also demonstrate acquisition of direction-dependent dark-field and differential phase-contrast images. From a pair of opposite line or rectangular illumination, structures along the illumination axis were enhanced in dark-field images and differential phase-contrast images. Raspberry-Pi is open-source hardware. Raspberry-Pi-based compact, cost-effective, and multi-contrast microscopes are expected to be useful for science, technology, engineering and mathematics (STEM) education.
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