Abstract
Chemical synthesis of Ag-NPs was carried out using reduction method. The reduction mechanistic approach of silver ions was found to be a basic clue for the formation of the Ag-NPs. The nanoparticles were characterized by UV-vis, FT-IR and TEM analysis. We had designed some experiments in support of our hypothesis, “low concentrations of novel nanoparticles (silver and gold) increases the activity of plant peroxidases and alter their structure also”, we had used Ag-NPs and HRP as models. The immobilization/interaction experiment had demonstrated the specific concentration range of the Ag-NPs and within this range, an increase in HRP activity was reported. At 0.08 mM concentration of Ag-NPs, 50% increase in the activity yield was found. The U.V-vis spectra had demonstrated the increase in the absorbance of HRP within the reported concentration range (0.06–0.12 mM). Above and below this concentration range there was a decrease in the activity of HRP. The results that we had found from the fluorescence spectra were also in favor of our hypothesis. There was a maximum increase in ellipticity and α-helix contents in the presence of 0.08 mM concentration of Ag-NPs, demonstrated by circular dichroism (CD) spectra. Finally, incubation of a plant peroxidase, HRP with Ag-NPs, within the reported concentration range not only enhances the activity but also alter the structure.
Highlights
Nanotechnology, an interdisciplinary research field involving chemistry, engineering, biology, and medicine, has great potential for early detection, accurate diagnosis and personalized treatment of cancer and other diseases [1]
The change in the color from white to yellow illustrates the formation of the silver nanoparticles with the peak at 400 nm and was further confirmed the formation of Ag-NPs by chemical reduction method
For the first time in the field of bio-nanotechnology, we have reported the immobilization of horseradish peroxidase (HRP) with different concentration of chemically formed Ag-NPs by reduction method
Summary
Nanotechnology, an interdisciplinary research field involving chemistry, engineering, biology, and medicine, has great potential for early detection, accurate diagnosis and personalized treatment of cancer and other diseases [1]. Nanoparticles (NPs) are typically smaller than several hundred nanometers in size, comparable to large biological molecules such as enzymes, receptors and antibodies. The most well studied nanoparticles are quantum dots [2,3], carbon nanotubes [4], paramagnetic nanoparticles [5], liposomes [6], gold nanoparticles [7] and many others [8]. It seems that these NPs might take center stage in many key future technologies because of recent efforts in fabricating these nanosized structures into predefined superstructures. There is a need to develop safe, reliable, nontoxic, cost effective, clean and ecofriendly methods for the preparation of NPs
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