Abstract
Expression of chicken and rat liver bifunctional enzyme, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, inEscherichia coliencountered two common problems: the chicken enzyme was liable to proteolysis and the rat enzyme was prone to form inclusion bodies. Reducing the rate of protein synthesis by lowering either growth temperature or isopropyl-β-d-thiogalactopyranoside (IPTG) concentration alleviated these two problems. Growth at 22°C was optimum for expression of both enzymes. The optimum range of IPTG concentration for expression was 0.1–1 μmfor the chicken liver bifunctional enzyme and 10 μmfor rat liver enzyme. The components of growth medium also influenced the production. Compared with Luria–Bertani medium, an enriched medium—tryptone–phosphate medium—tripled the production of the active enzymes. Addition of glucose (0.2%) doubled the expression level of active chicken liver enzyme, but reduced the production of active rat liver enzyme to half the maximal level, while the phosphate in tryptone–phosphate medium had no effect on the production of the two enzymes.
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